最新论文
[已阅读次]
无机药物化学课题组陈金香教授以通讯作者在Anal. Chim. Acta和Dalton Trans.上发表研究论文

无机药物化学课题组陈金香教授以通讯作者在Anal. Chim. Acta和Dalton Trans.上发表研究论文

近日,无机药物化学课题组陈金香教授分别在Anal. Chim. Acta和Dalton Trans.上发表了题为“A Zinc(II)-based two-dimensional MOF for sensitive and selective sensing of HIV-1 ds-DNA sequences”和“A zwitterionic 1D/2D polymer co-crystal and its polymorphic sub-components: a highly selective sensing platform for HIV ds-DNA sequences”的研究论文。

合成出系列金属有机骨架材料,从中筛选出了两个具有两维结构的金属有机骨架,能特异性的检测HIV-1 ds-DNA片段,检测限达到pM级,为金属有机骨架材料作为核酸分子的检测试剂提供了重要的指导。该类研究的报道受到了新科院士谭蔚泓教授的高度的关注,并发email表示祝贺“I enjoyed reading your paper. It is very nice to combine both imaging modalities in one nanoparticle. Congrats!!!!!”。

此两篇论文均受到广东省公益研究与能力建设专项资金、广东省自然科学基金的资助,论文第一作者均为2015届硕士研究生赵海清。Anal. Chim. Acta均为大类化学和小类分析化学2区杂志, IF=4.513;Dalton Trans.均为大类化学和小类无机化学与核化学2区杂志,IF=4.197。

Zhao, H. Q.#; Qiu, G. H.#; Liang, Z.; Li, M. M.; Sun, B.; Qin, L.; Yang, S. P.; Chen, W. H.; Chen,J. X.* A Zinc(II)-based two-dimensional MOF for sensitive and selective sensing of HIV-1 ds-DNA sequences, Anal. Chim. ACTA, 2016, DOI 10.1016/j.aca.2016.03.054.

Abstract

Coordination reaction of a known three-dimensional (3D) polymer precursor {Na3[Na9(Cbdcp)6(H2O)18]}n (A, Cbdcp = N-(4-carboxybenzyl)-(3,5-dicarboxyl)pyridinium) with Zn(NO3)2·6H2O in H2O or H2O/DMF at 100 °C and in the presence of aspirin, 5-fluorouracil (5-FU) as modulators, trans-1,2-bis(4-pyridyl)ethylene (bpe) or 1,2-bis(4-pyridyl)ethane (bpea) as ancillary ligands afforded six novel Zn(II)-based metal-organic frameworks (MOFs), that is, {[Zn(Cbdcp)(H2O)3]·H2O}n (1, 1D zigzag chain), {[Zn(HCbdcp)2]·H2O}n (2, 2D sheet), {[Zn(Cbdcp)(bpe)1/2]·2H2O}n (3, 3D polymer), {[Zn(Cbdcp)(bpe)1/2]·2H2O}n (4, 2D network), {[Zn(Cbdcp)(bpea)1/2]·2H2O}n (5, 3D polymer) and {[Zn(Cbdcp)(bpea)1/2]·2H2O}n (6, 2D network). Among them, compound 2 contains aromatic rings, positively charged pyridinium, Zn2+ cation centers and carboxylic acid groups lined up on the 2D sheet structure with a certain extended surface exposure. The unique structure of 2 facilitates effective association with carboxyfluorescein (FAM) labeled probe single stranded DNA (probe ss-DNA, delineates as P-DNA) to yield a P-DNA@2 system, and leads to fluorescence quenching of FAM via a photoinduced electron transfer process. The P-DNA@2 system is effective and reliable for the detection of human immunodeficiency virus 1 ds-DNA (HIV ds-DNA) sequences and capable of distinguishing complementary HIV ds-DNA from mismatched target sequences with the detection limit as low as 10 pM (S/N = 3).

Zhao, H. Q.#; Yang, S. P.#; Ding, N. N.; Qin, L.; Qiu, G. H.; Chen, J. X.*; Zhang, W. H.*; Chen, W. H.*; Hor, T. S. Andy*. A zwitterionic 1D/2D polymer co-crystal and its polymorphic sub-components: a highly selective sensing platform for HIV ds-DNA sequences, Dalton Trans., 2016, 45, 5092–5100.

Abstract

Polymorphic compounds {[Cu(dcbb)2(H2O)2]∙10H2O}n (2, 1D chain), [Cu(dcbb)2]n (3, 2D layer) and their co-crystal {[Cu(dcbb)2(H2O)][Cu(dcbb)2]2}n (4) have been prepared from the coordination reaction of a 2D polymer [Na(dcbb)(H2O)]n (1, H2dcbbBr = 1-(3,5-dicarboxybenzyl)-4,4’-bipyridinium bromide) with Cu(NO3)2·3H2O at different temperatures in water. Compounds 2−4 have identical metal-to-ligand stoichiometric ratio of 1:2, but absolutely differ in structure. Compound 3 features a 2D layer structure with aromatic rings, positively charged pyridinium and free carboxylates on its surface, promoting electrostatic, π-stacking and/or hydrogen-bonding interactions with the carboxyfluorescein (FAM) labeled probe single-stranded DNA (probe ss-DNA, delineates as P-DNA). The resultant P-DNA@3 system facilitated fluorescence quenching of FAM via a photoinduced electron transfer process. The P-DNA@3 system functions as an efficient fluorescent sensor selective for HIV double-stranded DNA (HIV ds-DNA) due to the formation of rigid triplex structure with the recovery of FAM fluorescence. The system reported herein also distinguishes complementary HIV ds-DNA from mismatched target DNA sequences with the detection limit of 1.42 nM.