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刘瑞源副教授以共同通讯作者在J. Mater. Chem. B上发表研究论文

刘瑞源副教授以共同通讯作者在J. Mater. Chem. B上

发表研究论文

近日,刘瑞源副教授以共同通讯作者在J. Mater. Chem. B上发表题为“Poly(phenyleneethynylene) nanoparticles: preparation, living cell imaging and potential application as drug carriers”研究论文。

该研究利用新型亲水亲油两亲性聚(亚苯基亚乙炔基)自组装构建共轭聚合物纳米粒子(PPE纳米粒子)。检测PPE纳米粒子的形貌和细胞毒性。激光共聚焦显微镜和流式细胞术结果显示肿瘤细胞可以有效内吞PPE纳米粒子。PPE纳米粒子还可以应用为药物载体。PPE纳米粒子显示出高载药量(26.6%)和良好的药物释放性能。采用DLS和TEM表征载阿霉素PPE纳米粒子的形态。最后,细胞内吞,细胞毒性和Western Blotting结果表明PPE纳米粒子成功地将药物传递进入肿瘤细胞,并且有效地保持阿霉素的抗肿瘤活性。所有研究结果显示新型PPE纳米粒子可以应用为药物载体和细胞荧光成像。

Dalton Trans.均为大类工程和小类生物材料1区杂志,IF=4.279。

Tong Chen, Wanfu Xu, Zehai Huang, Hongmei Peng, Zhiyong Ke,* Xinwei Lu, Yichen Yan and Ruiyuan Liu*,Poly(phenyleneethynylene) nanoparticles: preparation, living cell imaging and potential application as drug carriers,J. Mater. Chem. B, 2015, 3, 3564-3572

Abstract

Conjugated polymer nanoparticles (PPE nanoparticles) are fabricated by the self-assembly of novel amphiphilic poly(phenyleneethynylenes). The morphology and cytotoxicity of PPE nanoparticles were investigated. Moreover, confocal fluorescence microscopy and flow cytometry assay revealed the effective internalization of PPE nanoparticles. PPE nanoparticles were also used as a carrier for drug delivery systems. Upon encapsulating an anticancer drug, DOX, PPE nanoparticles exhibited high drug loading efficiency (26.6 wt%) and good release properties. Subsequently, DOX loaded PPE nanoparticles were investigated by employing dynamic light scattering and transmission electron microscopy. Finally, cell uptake, cytotoxicity and Western blotting analysis were carried out, which revealed that PPE nanoparticles successfully deliver the drug into cancer cells and retain the anticancer bioactivity of DOX. All these results indicated that this new type of PPE nanoparticles would be a promising drug delivery system for therapeutic delivery and/or bioimaging applications.