南方医科大学法医学院法医精神病学系研究方向及研究成果

一、研究方向

（1）毒品和NPS精神损伤快速鉴识、成瘾机制及防治。

（2）法医精神病人工智能鉴识、伪装精神障碍机制研究与评定技术开发。

（3）精神损伤所致精神障碍的机制研究与评定技术开发。

（4）脑损伤导致认知及情绪障碍的神经机制探索及鉴定技术开发。

（5）法医基因组学应用。

二、研究成果

1. 学术论文

（1）Huang L, Du J, Ye L, Zheng Y, Liu X, Huang E, Le J, Huang X, Du W, Liu C, Chen L. Species level and SNP profiling of skin microbiome improve the specificity in identifying forensic fluid and individual. Forensic Sci Int Genet. 2025 Jun;78:103256. doi: 10.1016/j.fsigen.2025.103256. Epub 2025 Mar 4. PMID: 40073753.

（2）Ye L, Zhang X, Liu X, Huang L, Chen X, Zheng Y, Du J, Lun M, Xu Q, Du W, Liu C, Chen L. A multiplex bacterial assay for identifying vaginal fluid in various individual states and mixture stains. Forensic Sci Int Genet. 2025 Jun;78:103304. doi: 10.1016/j.fsigen.2025.103304. Epub 2025 May 27. PMID: 40449284.

（3）Yubo Hu; Zhe Li; Mengdan Xing, Xiaoru Xie, Panwu Zhao; Xin Cheng; Chuan Xiao; Yuting Xia; Jingru Wu; Yuan Luo; Ho Ko; Yamei Tang; Xiaojing Ye; Wei-Jye Lin; Microglial repopulation reverses radiation-induced cognitive dysfunction by restoring medial prefrontal cortex activity and modulating leukotriene-C4 synthesis, Acta Neuropathologica Communications, 2025

（4）Yu-Bo Hu; Xi Deng; Lu Liu; Can-Can Cao; Ya-Wen Su; Zhen-Jie Gao; Xin Cheng; Deshan Kong; Qi Li; Yan-Wei Shi; Xiao-Guang Wang; Xiaojing Ye; Hu Zhao ; Distinct roles of excitatory and inhibitory neurons in the medial prefrontal cortex in the expression and reconsolidation of methamphetamine-associated memory in male mice, Neuropsychopharmacology, 2024

（5）Huang L, Huang H, Liang X, Su Q, Ye L, Zhai C, Huang E, Pang J, Zhong X, Shi M, Chen L. Skin locations inference and body fluid identification from skin microbial patterns for forensic applications. Forensic Sci Int. 2024 Sep;362:112152. doi: 10.1016/j.forsciint.2024.112152. Epub 2024 Jul 22. PMID: 39067177.

（6）Huang L, Liang X, Xiao G, Du J, Ye L, Su Q, Liu C, Chen L. Response of salivary microbiome to temporal, environmental, and surface characteristics under in vitro exposure. Forensic Sci Int Genet. 2024 May;70:103020. doi: 10.1016/j.fsigen.2024.103020. Epub 2024 Jan 24. PMID: 38286081.

（7）Zheng Y, Ye L, Du J, Huang L, Lun M, He M, Xiao G, Du W, Liu C, Chen L. Changes in the microbial community of semen exposed to different simulated forensic situations. Microbiol Spectr. 2024 Aug 6;12(8):e0012524. doi: 10.1128/spectrum.00125-24. Epub 2024 Jul 9. PMID: 38980015; PMCID: PMC11302308.

（8）Liu X, Yang C, Chen X, Han X, Liu H, Zhang X, Xu Q, Yang X, Liu C, Chen L, Liu C. A novel 193-plex MPS panel integrating STRs and SNPs highlights the application value of forensic genetics in individual identification and paternity testing. Hum Genet. 2024 Mar;143(3):371-383. doi: 10.1007/s00439-024-02658-1. Epub 2024 Mar 18. PMID: 38499885.

（9）Du W, Liu X, Huang L, Zheng Y, Wu W, Huang Q, Li T, Wei R, Yang Q, Deng S, Liao J, Liu C, Chen L. Developmental validation of a novel multiple genotyping assay with 24 Canine STR loci. Vet Q. 2023 Dec;43(1):1-18. doi: 10.1080/01652176.2023.2262521. Epub 2023 Oct 20. PMID: 37746714; PMCID: PMC10591528.

（10）Liang X, Liu X, Ye L, Du W, Huang L, Liu C, Xiao G, Huang M, Zheng Y, Shi M, Liu C, Chen L. Development and application of a multiplex PCR system for forensic salivary identification. Int J Legal Med. 2023 Jul;137(4):961-969. doi: 10.1007/s00414-023-03004-2. Epub 2023 May 2. PMID: 37127761.

（11）Yang C, He M, Liu C, Liu X, Lun M, Su Q, Han X, Liu H, Wang M, Chen L, Liu C. Development and validation of a custom panel including 114 InDels using massively parallel sequencing for forensic application. Electrophoresis. 2023 Nov;44(21-22):1704-1713. doi: 10.1002/elps.202300044. Epub 2023 Aug 25. PMID: 37622566.

（12）Liao L, Ye L, Huang L, Yao T, Liang X, Chen L, Shen M. Changes in the microbial community after vaginal fluid exposure in different simulated forensic situations. Forensic Sci Int. 2023 Aug;349:111766. doi: 10.1016/j.forsciint.2023.111766. Epub 2023 Jun 14. PMID: 37339565.

（13）Liao L, Sun Y, Huang L, Ye L, Chen L, Shen M. A novel approach for exploring the regional features of vaginal fluids based on microbial relative abundance and alpha diversity. J Forensic Leg Med. 2023 Nov;100:102615. doi: 10.1016/j.jflm.2023.102615. Epub 2023 Nov 10. PMID: 37995431.

（14）Liu X, Du W, Wang C, Wu Y, Chen W, Zheng Y, Wang M, Liu H, Yang Q, Qian S, Chen L, Liu C. A multilocus DNA mini-barcode assay to identify twenty vertebrate wildlife species. iScience. 2023 Oct 19;26(11):108275. doi: 10.1016/j.isci.2023.108275. PMID: 38026223; PMCID: PMC10651681.

（15）Zeng Y, Chen L, Wang M, Yang C, Liu H, Xiao C, Liu C, Li Y, Xu Q, Du W, Liu C. The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction. Front Genet. 2022 Jan 27;13:842004. doi: 10.3389/fgene.2022.842004. PMID: 35154290; PMCID: PMC8829124.

（16）Li B, Chen R, Chen L, Qiu P, Ai X, Huang E, Huang W, Chen C, Liu C, Lin Z, Xie WB, Wang H. Effects of DDIT4 in Methamphetamine-Induced Autophagy and Apoptosis in Dopaminergic Neurons. Mol Neurobiol. 2017 Apr;54(3):1642-1660. doi: 10.1007/s12035-015-9637-9. Epub 2016 Feb 12. PMID: 26873849.

（17）Zhu LN, Qiao HH, Chen L, Sun LP, Hui JL, Lian YL, Xie WB, Ding JY, Meng YL, Zhu BF, Qiu PM. SUMOylation of Alpha-Synuclein Influences on Alpha-Synuclein Aggregation Induced by Methamphetamine. Front Cell Neurosci. 2018 Aug 24;12:262. doi: 10.3389/fncel.2018.00262. PMID: 30197588; PMCID: PMC6117395.

（18）Sun L, Lian Y, Ding J, Meng Y, Li C, Chen L, Qiu P. The role of chaperone-mediated autophagy in neurotoxicity induced by alpha-synuclein after methamphetamine exposure. Brain Behav. 2019 Aug;9(8):e01352. doi: 10.1002/brb3.1352. Epub 2019 Jul 9. PMID: 31286692; PMCID: PMC6710200.

（19）Huang E, Huang H, Guan T, Liu C, Qu D, Xu Y, Yang J, Yan L, Xiong Y, Liang T, Wang Q, Chen L. Involvement of C/EBPβ-related signaling pathway in methamphetamine-induced neuronal autophagy and apoptosis. Toxicol Lett. 2019 Sep 15;312:11-21. doi: 10.1016/j.toxlet.2019.05.003. Epub 2019 May 3. PMID: 31059759.

（20）Chen L, Huang E, Wang H, Qiu P, Liu C. RNA interference targeting α-synuclein attenuates methamphetamine-induced neurotoxicity in SH-SY5Y cells. Brain Res. 2013 Jul 12;1521:59-67. doi: 10.1016/j.brainres.2013.05.016. Epub 2013 May 18. PMID: 23688541.

（21）Qiao HH, Zhu LN, Wang Y, Hui JL, Xie WB, Liu C, Chen L, Qiu PM. Implications of alpha-synuclein nitration at tyrosine 39 in methamphetamine-induced neurotoxicity in vitro and in vivo. Neural Regen Res. 2019 Feb;14(2):319-327. doi: 10.4103/1673-5374.244795. PMID: 30531016; PMCID: PMC6301162.

（22）Zhang F, Chen L, Liu C, Qiu P, Wang A, Li L, Wang H. Up-regulation of protein tyrosine nitration in methamphetamine-induced neurotoxicity through DDAH/ADMA/NOS pathway. Neurochem Int. 2013 Jun;62(8):1055-64. doi: 10.1016/j.neuint.2013.03.016. Epub 2013 Apr 11. PMID: 23583342.

（23）Tai Y, Chen L, Huang E, Liu C, Yang X, Qiu P, Wang H. Protective effect of alpha-synuclein knockdown on methamphetamine-induced neurotoxicity in dopaminergic neurons. Neural Regen Res. 2014 May 1;9(9):951-8. doi: 10.4103/1673-5374.133146. PMID: 25206917; PMCID: PMC4146216.

（24）Xu X, Huang E, Luo B, Cai D, Zhao X, Luo Q, Jin Y, Chen L, Wang Q, Liu C, Lin Z, Xie WB, Wang H. Methamphetamine exposure triggers apoptosis and autophagy in neuronal cells by activating the C/EBPβ-related signaling pathway. FASEB J. 2018 Jun 25:fj201701460RRR. doi: 10.1096/fj.201701460RRR. Epub ahead of print. PMID: 29939784.

（25）Wang JY^#, Li Q^#, Liu W, Yang Y, Wang XG, Liu CY, Shu XJ, Xue L*, Shi YW*. Posttraumatic stress disorder symptoms among Chinese college students following the COVID-19 outbreak,Front Neurosci，2023, 17:1075272.

（26）Li Q^#, Liu W^#, Wang JY^#, Wang XG, Hao B, Hu YB, Deng X, Liu L, Zhao H*, Shi YW*, Xue L*, Prevalence and risk factors of post-traumatic stress disorder symptoms among Chinese health care workers following the COVID-19 pandemic, Heliyon, 2023, 9(4):e14415.

（27）Liu XH^#, Zhu RT, Hao B, Shi YW, Wang XG, Xue L*, Zhao H*, Norepinephrine induces PTSD-like memory impairments via regulation of the β-adrenoceptor -cAMP/PKA and CaMK II/PKC systems in the basolateral amygdala, Front Behav Neurosci, 2019,13(43): 1-12.

（28）Zhu RT^#, Liu XH^#, Shi YW, Wang XG, Xue L*, Zhao H*, Propranolol can induce PTSD-like memory impairments in rats, Brain Behav, 2018, 8(2): e00905.

（29）Sun XM, Tu WQ, Shi YW, Xue L2*, Zhao H*, Female-dependent impaired fear memory of adult rats induced by maternal separation, and screening of possible related genes in the hippocampal CA1.Behav Brain Res, 2014,267:111-118.

（30）Xue L^#,Li ZD^#, Chen ZX, Wang XG, Shi YW*, Zhao H*, Fear response failed to return in AAB extinction paradigm accompanied with increased NR2B and GluR1 per845 in hippocampal CA1, Neuroscience, 2014,260:1-11.

（31）Chen ZX^#, Xue L^#, Liang CY, Wang LL, Mei W, Zhang Q, Zhao H*, Specific marker of feigned memory impairment: The activation of left superior frontal gyrus., J Forensic Leg Med, 2015, 36: 164-171.

（32）Du W, Liu X, Huang L, Zheng Y, Wu W, Huang Q, Li T, Wei R, Yang Q, Deng S, Liao J, Liu C, Chen L. Developmental validation of a novel multiple genotyping assay with 24 Canine STR loci. Vet Q. 2023 Dec;43(1):1-18. doi: 10.1080/01652176.2023.2262521. Epub 2023 Oct 20. PMID: 37746714; PMCID: PMC10591528.

（33）Ye L, Liao L, Lan J, Huang L, Du J, Zhang X, Lun M, Zhu B, Liu C, Chen L. Temporal dynamics changes in the vaginal fluid microbiome: Implications for body fluid identification and estimating time since deposition (TsD) for forensics. Forensic Sci Int. 2024 Nov;364:112219. doi: 10.1016/j.forsciint.2024.112219. Epub 2024 Sep 10. PMID: 39270472.

（34）Wang, N., Ou, Y., Wang, Y., Huang, Z., Hu, Y., & Zhao, S. (2026). Xylazine-Adulterated Fentanyl-Induced Brain Toxicity: Mechanism Elucidation via Network Toxicology and Molecular Docking Approaches. J Appl Toxicol, 46(1), 354-361. doi:10.1002/jat.4902

（35）Yingyi Luan#, Enping Huang#, Jiajia Huang, Zhenjia Yang, Zhipeng Zhou, Yan Liu, Conglin Wang, Ming Wu*. Serum myoglobin modulates kidney injury via inducing ferroptosis after exertional heatstroke. J Transl Int Med, 2023, 11(2):178-188.

（36）Huang E#, Huang H#, Guan T, Liu C, Qu D, Xu Y, Yang J, Yan L, Xiong Y, Liang T, Wang Q*, Chen L*. Involvement of C/EBPβ-related signaling pathway in methamphetamine-induced neuronal autophagy and apoptosis. Toxicol Lett, 2019, 312:11-21.

（37）Huang E#, Guan T#, Li L, Xia P, Peng Z, Feng C, Chen L*, Genetic polymorphisms of 20 STR loci in a Chaoshan group from Jieyang, China, Leg Med (Tokyo), 2019, 36:41-42.

（38）Xu X#, Huang E#, Luo B#, Cai D, Zhao X, Luo Q, Jin Y, Chen L, Wang Q, Liu C, Lin Z, Xie WB*, Wang H*, Methamphetamine exposure triggers apoptosis and autophagy in neuronal cells by activating the C/EBPβ-related signaling pathway, FASEB J, 2018, 32(12): 6737-6759.

（39）Li K#, Huang EP#, Su J, Zhu P, Lin J, Luo SQ, Yang CL*, Therapeutic Role for TSP-2 Antibody in a Murine Asthma Model, Int Arch Allergy Immunol, 2018, 175(3):160-170.

（40）Xu X#, Huang E#, Tai Y, Zhao X, Chen X, Chen C, Chen R, Liu C, Lin Z, Wang H*, Xie WB*, Nupr1 Modulates Methamphetamine-Induced Dopaminergic Neuronal Apoptosis and Autophagy through CHOP-Trib3-Mediated Endoplasmic Reticulum Stress Signaling Pathway, Front Mol Neurosci, 2017, 10: 203.

（41）Chen L#, Huang E#, Wang H, Qiu P*, Liu C*. RNA interference targeting α-synuclein attenuates methamphetamine-induced neurotoxicity in SH-SY5Y cells. Brain Res. 2013, 1521:59-67.

2. 科研项目（纵向+横向，包含人才项目）

（1）国家自然科学基金面上项目：基于mNGS绝对定量技术解析体液斑微生物组的时相变化特征及其法医学应用研究(编号82471919)，2025.01-2028.12，50万，主持人，在研。

（2）国家自然科学基金面上项目：基于16S rDNA微单倍型标记的混合斑体液识别和身份溯源研究(编号82271927)，2023.01-2026.12，53万，主持人，在研。

（3）国家重点研发计划项目子课题：新精神活性物质快速鉴识与动态预警技术研究-基于中医疗法的新精神活性物质滥用戒治新模式及机制研究（编号No.2024YFC3306605），2025.05-2028.04，130万，主持人及核心骨干，在研。

（4）涉密项目子课题：XXX（编号XXX），2025.08-2028.07，100万，主持人，在研。

（5）广东省科技厅，广东省基础与应用基础研究重大项目，基于神经细胞互作的抗抑郁新靶标与新型诊疗策略研究，子课题负责人，2025/09至2030/08，66万，主持，在研

（6）广东省自然科学基金面上项目：腹侧背盖区-内侧前额叶皮质神经环路调控甲基苯丙胺成瘾记忆的神经机制研究（2025A1515010509）, 2025.01-2027.12, 10万，在研, 主持

（7）广东省自然科学基金面上项目，C/EBPβ介导的铁死亡在甲基苯丙胺诱导的神经毒性中的分子机制及法医学应用研究（2025A1515012726），2025.01-2027.12，10万元，主持，在研

（8）国家自然科学基金青年基金项目，转录因子C/EBPβ在甲基苯丙胺诱导的神经毒性损伤中的作用及分子机制研究（81801876），2019.01-2021.12，21万元，主持，结题。

（9）国家自然科学基金青年项目：DDIT4-mTOR信号轴介导的自噬在METH神经毒性损伤中的作用及其机制（编号：81501627），2016.01-2018.12，21.6万，主持人，结题。

（10）广东省自然科学基金面上项目：微生物标记用于法医学体液斑沉积时间推断的探索研究(编号2022A1515012011)，2024.01-2026.12，15万，主持人，在研。

（11）广州市科技计划项目：基于微生物的腐败尸体死亡原因和死亡时间推断技术研究(编号2024B04J0022)，2024.11-2027.10，主持人，在研。

（12）广州市科技计划项目，黄芩素通过STIM1介导的铁死亡干预甲基苯丙胺神经毒性的机制研究（202201011630），2022.04-2024.03，5万元，主持，结题，

（13）广东省自然科学基金：人体皮肤微生物标记的甄选和用于法医组织体液溯源的效能评估（2022A1515012011），2022.01-2024.12，10万，主持，结题。

（14）广东省自然科学基金：微生物群落用于人体组织体液溯源的探索研究，广东省自然科学基金（2020A1515010938）2019.01-2022.12，10万，主持，结题。

（15）国家自然科学基金委员会，青年基金，81601652，海马-前额叶通路对条件性恐惧记忆特异性与泛化的调控机制研究，2017/01至2019/12，17万元，主持，结题

（16）中国博士后科学基金委员会，特别资助项目，2015T80929，恐惧记忆特异性与泛化：海马-前额叶通路调控机制研究，2016/01至2018/12，15万元，主持，结题

（17）广东省自然科学基金委员会，博士启动项目，2014A030310089，PTSD样记忆损害形成中糖皮质激素的快速调控机制研究，2015/01至2018/01，10万元，主持，结题

（18）中国博士后科学基金委员会，面上项目，2014M562229，糖皮质激素在PTSD样记忆损害形成中的快速调控机制研究，2014/07至2016/06，5万元，主持，结题

3. 成果奖励（科研、教学）

（1）2025 年学校优秀教学成果奖（高等教育-本科类）二等奖，第一完成人；

（2）2022年公安部科学技术二等奖；

（3）2020年广东省科技进步一等奖；

（4）2013年广东省科技进步二等奖；

（5）2012年广州市科技进步一等奖；

4. 授权专利

（1）中国发明专利授权：《伪装认知功能障碍的fMRI鉴定方法》（专利号：ZL201410623580X）。

5. 标准制定

（1）参编《精神障碍者受审能力评定指南》（SF/Z JD0104005—2018）