林瑛 博士

副教授

电子邮件: linying0216@smu.edu.cn

电 话：020-62789442

通讯地址：广东省广州市白云区沙太南路1023（510515）

个人简介

林瑛，副教授，北京大学生物化学与分子生物学博士，美国加州大学圣地亚哥分校（UCSD）博士后。主要研究方向为基因编辑与肺类器官的疾病模拟，重点研究干细胞与肺部疾病的关系。主持和参与国家科技重大专项，国家自然科学基金重点和面上项目，广东省自然等多项课题，在Nucleic Acids Research, Protein & Cell, Stem Cell Research and Therapy，Communications Biology，J Gene Med，Cancer Medicine等期刊发表SCI论文19篇，申请PCT国际专利1项和中国发明专利6项（4项已授权）。

研究方向

基因编辑与肺类器官的疾病模拟

Gene Editing and Lung Organoids for Disease Modeling

研究关键词

Gene editing, Lung organoids

代表性论著

1. Ma, S.^#, Lv, J.^#, Feng, Z.^#, Rong, Z.^*, Lin, Y.^* Get ready for the CRISPR/Cas system: A beginner's guide to the engineering and design of guide RNAs. J Gene Med, e3377. (2021)

2. Huang, H. ^#, Hu, Y. ^#, Huang, G., Ma, S., Feng, J., Wang, D., Lin, Y^*., Zhou, J^*., Rong, Z.^* Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases. Communications Biology 4, 830 (2021)

3. Li, L.^#, Feng, J.^#, Zhao, S., Rong, Z.^*& Lin, Y^*. SOX9 inactivation affects the proliferation and differentiation of human lung organoids. Stem Cell Research and Therapy 12, 343 (2021)

4. Ma, S., Wang, X., Hu, Y., Lv, J., Liu, C., Liao, K., Guo, X., Wang, D., Lin, Y.^* & Rong, Z. ^* Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain. Nucleic Acids Res 48, 10590-10601 (2020).

5. Huang, H.^#, Zhang, X.^#, Lv, J., Yang, H., Wang, X., Ma, S., Shao, R., Peng, X., Lin, Y.^* & Rong, Z.^* Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system. Protein & cell 11, 299-303 (2020).

6. Yang H#; Shao R#; Huang H#; Wang X; Rong Z, Lin Y*, Engineering macrophages to phagocytose cancer cells by blocking the CD47/SIRPɑ axis, Cancer Medicine, 8(9): 4245-4253 (2018) (Cover story)

7. Zhang, X.^#, Wang, W.^#, Shan, L., Han, L., Ma, S., Zhang, Y., Hao, B., Lin, Y.^* & Rong, Z.^* Gene activation in human cells using CRISPR/Cpf1-p300 and CRISPR/Cpf1-SunTag systems. Protein & cell 9, 380-383 (2018).

8. Ma, S., Lv, J., Sun, J., Tang, P., Li, H., Zhou, H., Zhang, Z., Lin, Y.^* & Rong, Z.^* iKA-CRISPR hESCs for inducible and multiplex orthogonal gene knockout and activation. FEBS letters 592, 2238-2247 (2018).

9. Chen, Y., Feng, J., Zhao, S., Han, L., Yang, H., Lin, Y.^* & Rong, Z.^* Long-Term Engraftment Promotes Differentiation of Alveolar Epithelial Cells from Human Embryonic Stem Cell Derived Lung Organoids. Stem cells and development 27, 1339-1349 (2018). (Cover story)

10. Ye, H., Rong, Z.^* & Lin, Y.^* Live cell imaging of genomic loci using dCas9-SunTag system and a bright fluorescent protein. Protein & cell 8, 853-855 (2017).

代表性课题

1. 广东省基础与应用基础研究面上项目，2022.01-2024.12，主持，课题经费10万

2. 国家自然科学基金面上项目，2021.01-2024.12，主持，课题经费55万元

3. 广东省自然科学基金自由申请，2018.05-2021.04，主持， 课题经费10万元

4. 广东省自然科学基金-博士启动纵向协同，2017.01-2019.12，主持，课题经费 10万元

5. 广州再生医学与健康广东省实验室前沿探索项目，2018/12-2021/12，参与，课题经费 150万元

6. “艾滋病和病毒性肝炎等重大传染病防治”国家科技重大专项，2018/01-2020/12，参与，课题经费120.21万元

7. 国家自然科学基金面上项目，2017/01-2019/12，参与，课题经费60万元