主要科研方向有4个：
- 新发、再现、机会性致病寄生虫的新型诊治技术研究：包括重要诊断性及保护性抗原的基因工程制备，应用重组抗原构建诊断试剂盒，研制基因工程疫苗、DNA疫苗等。主要的防控研究对象为弓形虫、日本血吸虫、广州管圆线虫等。
- 蚊媒的分子生物学及防护技术研究：运用现代分子生物学、高分子材料、控释缓释、驱杀虫剂复配等技术手段来研究蚊媒的分子生物学特性和蚊媒的个人及集体防护方法，包括：蚊媒重要基因的分离克隆，转基因蚊虫的构建，登革感染引起蚊媒miRNA谱的变化，蚊媒的长效、高效驱杀虫药械的研制等。
- 病原的表观遗传学及病原与宿主细胞的相互作用研究：研究弓形虫Pru株速殖子和缓殖子基因组的甲基化、组蛋白的甲基化和乙酰化水平，从表观遗传学角度解释速殖子与缓殖子的相互转化机制。研究弓形虫感染宿主细胞后细胞信号网络发生的变化，进而从细胞信号水平解释弓形虫感染的机制；及研究登革病毒感染肝细胞后，细胞信号网络发生的变化，从细胞信号水平研究登革感染所引起的肝损伤的机制。
- 生物杀虫剂的开发：利用昆虫病毒和真菌对昆虫特异性感染的特点，将外源毒力基因引入并构建重组昆虫病毒和真菌以提高其致病性，主要应用于蚊媒的防控。
A Brief introduction for Department of Pathogen Biology
Department of Pathogen Biology is the basis of the Key Laboratory of Prevention and Control for Emerging Infectious Diseases of Guangdong Higher Institutes, and the key Laboratory of Tropical diseases of Guangdong Province. The course of “Medical Parasitology”taught by this department is the “National Essence Course”, “National Bilingual Teaching Demonstration Course” and “Graduates Demonstration Course of Guandong Province”.
In this five years, this department has got more than 10 million Yuan funding support from NIH, USA (430,000 USD), Guangdong Province Leading Talent Project (6 million Yuan), National Natural Science Foundation of China and Guangdong province.
The research directions of this department are as follows.
- The research of new diagnostic and prevention method for new-emerging, re-emerging, and opportunistic parasitosis: the diagnostic and protective antigens were screened and prepared in large scale. The recombinant diagnostic antigens were used for diagnostic kit construction. Genetic and DNA vaccines are also exploited. The pathogen targets includes Toxoplasma gondii,Schistosoma japonicum, and Angiostrongylus cantonensis.
- The research of molecular biology and preventive methods of mosquito vectors: modern molecular biology, high molecular material, releasing control and recipes of pesticide and repellent research are applied to research on the mosquito molecular biology characteristics and personal/mass preventive methods, including gene cloning, transgenic mosquito platform construction, miRNA profile change of mosquito infected by dengue virus and long lasting repellent exploit.
- The research of pathogen epigenetics and the pathogen-host cell interaction: T. gondii Pru tachyzoites and bradyzoites genome methylation, histone methylation and acylation levels are detected to explain the mechanism of the conversion between the tachyzoite and bradyzoite. The cell signaling network change of the host cell after infection by T. gondii was analyzed to explain the infection mechanism of this parasite; The cell signaling network change of liver cells after infection by different serum type of dengue viruses was analyzed to explain the mechanism of liver injury.
- Bio-insecticides exploitation: the specific infection characteristics of insect viruses and fungi were utilized, and some toxin genes were introduced into to construct recombinant insect viruse and fungus to improve their pathogenic ability. The recombinant insect viruse and fungus are mainly used in mosquto control.
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1 代表性课题 |
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2 代表性论文： |
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1. Wang C, Xu J, Zhou X, Li J, Yan G, James AA, Chen XG (通讯作者). Strongyloidiasis: an emerging infectious disease in China. Am J Trop Med Hyg. 2013 Mar;88(3):420-5. doi: 10.4269/ajtmh.12-0596. IF: 2.592 2. Gu J, Hu W, Wu J, Zheng P, Chen M, James AA, Chen XG(通讯作者). , Tu Z. miRNA genes of an invasive vector mosquito, Aedes albopictus. PLoS One. 2013 Jul 1;8(7):e67638. doi: 10.1371/journal.pone.0067638. Print 2013. IF:3.73 3. Deng Y, Yan H, Gu J, Xu J, Wu K, Tu Z, James AA, Chen XG (通讯作者) .Molecular and functional characterization of odorant-binding protein genes in an invasive vector mosquito, Aedes albopictus. PLoS One. 2013 Jul 23;8(7):e68836. doi: 10.1371/journal.pone.0068836. Print 2013. IF:3.73 4. Puthiyakunnon S, Yao Y, Li Y, Gu J, Peng H, Chen XG Chen XG (通讯作者). Functional characterization of three MicroRNAs of the Asian tiger mosquito, Aedes albopictus. Parasit Vectors. 2013 Aug 8;6(1):230. doi: 10.1186/1756-3305-6-230. IF: 3.246 5. Na RH, Zhu GH, Luo JX, Meng XJ, Cui L, Peng HJ(通讯作者), Chen XG (通讯作者), Gomez-Cambronero J.Enzymatically active Rho and Rac small-GTPases are involved in the establishment of the vacuolar membrane after Toxoplasma gondii invasion of host cells.BMC Microbiol. 2013 May 30;13:125. doi: 10.1186/1471-2180-13-125. IF: 3.10 6. 7.Wu XB,Na RH, Wei SS,Zhu JS, Peng HJ(通讯作者).Distribution of Tick-borne Diseases in China. Parasites & Vectors. 2013, 6:119 doi:10.1186/1756-3305-6-119 IF:3.25 7. Tan F, Hu X, Luo FJ, Pan CW, Chen XG(通讯作者). .Induction of protective Th1 immune responses in mice by vaccination with recombinant Toxoplasma gondii nucleoside triphosphatehydrolase-II.Vaccine. 2011 Mar 24;29(15):2742-8. 8. Gao XJ, Zhao ZJ, He ZH, Wang T, Yang TB, Chen XG, Shen JL, Wang Y, Lv FL, Hide G, Lun ZR.Toxoplasma gondii infection in pregnant women in China.Parasitology. 2012 Feb;139(2):139-47. 9. Ren J, Feng Z, Lv Z, Chen XG, Li J. Natural Killer-22 Cells in the Synovial Fluid of Patients with Rheumatoid Arthritis Are an Innate Source of Interleukin 22 and Tumor Necrosis Factor-{alpha}.J Rheumatol. 2011,38(10)：2112-8. 10. Cui LW*, Yan GY, Sattabongkot J, Cao YM, Chen B, Chen XG, Fan Q, Fang Q, Jongwutiwes S, Parker D, Sirichaisinthop J, Kyaw MP, Su XZ, Yang HL, Yang ZQ, Wang BM, Xu JW, Zheng B, Zhong BB, Zhou GF. Malaria in the Greater Mekong Subregion: Heterogeneity and complexity.Acta Tropica. 2012 Mar;121(3):227-39. 11. Liu CY, Song HQ, Zhang RL, Chen MX, Xu MJ, Ai L, Chen XG, Zhan XM, Liang SH, Yuan ZG, Lin RQ*, Zhu XQ*. Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay. Molecular and Cellular Probes. 2011,25：164-167. 12. Zhang H., Zhou YP., Ding HJ., Wang WJ., Zhao W., Hou JL, Chen XG. The clinical characteristics and epidemiological outbreak in Dongguan City Southern China.International Journal of Infectious Diseases. 2011,15(S1)：S113. 13. Liu M, Wang T, Li H, Li JY, Zhong H, Tan CZ, Huang DC, Zhao L, Chen XG*, Lun ZR *. Analysis of the antibodies anti-Toxoplasma gondii by ELISA based on two diagnostic antigens: rSAG1 and rBAG1. Acta Parasitologica. 2011,56(4)：353-359 14. Peng H-J *, Chen XG *, and Lindsay D-S. A Review: Competence, Compromise, and Concomitance—Reaction of the Host Cell To Toxoplasma gondii Infection and Development. J. Parasitol., 2011,97( 4 )：620-628. 15. Peng H-J, Karen M. Henkels, Madhu Mahankali,Marchal C, Dinauer MC. and Gomez-Cambronero J. Evidence For Two Crib Domains In Phospholipase D2 (Pld2) That The Enzyme Uses to Specifically Bind to The Small Gtpase Rac2. J Bio.Chem.2011,286(18): 16308-20 IF:5.328 16. Peng H-J, Henkels KM,Farkaly T Post, Frondorf K, Bubulya P and Gomez-Cambronero J. The dual effect of Rac2 on phospholipase D2 regulation that explains both the onset and termination of chemotaxis. Mol Cell Biol. 2011;31(11):2227-40. IF:6.188 17. Peng HJ, Lai HB, Zhang QL, Xu BY, Zhang H, Liu WH, Zhao W, Zhou YP, Zhong XG, Jiang S, Duan JH, Yan GY, He JF, Chen XG. A local outbreak of dengue caused by an imported case in Dongguan China.BMC Public Health. 2012;12(1):83 IF:2.36 18. Peng H-J, CHEN X-G, Lun Z-R. Analysis of the gene expression profile of Schistosoma japonicum cercariae by expressed sequence tag strategy. Parasitology Research. 2003, 90(4):287-293 IF:1.812 19. Madhu Mahankali, Peng H-J, Karen M. Henkels, Mary C. Dinauer, and Julian Gomez-Cambronero. Phospholipase D2 (PLD2) Q:1 is a guanine nucleotide exchange factor (GEF) for the GTPase Rac2.PNAS;2011;108(49):19617-22. IF: 9.56 20. Madhu Mahankali, Peng H-J,COX D, Gomez-Cambronero J.The Mechanism Of Cell Membrane Protrusion Formation Relies On A Phospholipase D2 (Pld2), Grb2 And Rac2 Association. Cell Signalling. 2011 Aug;23(8):1291-8. Epub 2011 Mar 16. IF:4.23 21. Henkels KM, Short S, Peng H-J, Gomez-Cambronero J. 2009.PLD2 has both enzymatic and cell proliferation- inducing capabilities, that are differentially regulated by phosphorylation. Biochem. Biophys.Res.Commun. 389(2):224-228 IF: 2.595 22. Henkels KM, Peng H-J, Frondorf K, Gomez-Cambronero J.2010. A comprehensive model that explains the regulation of Phospholipase D2 (PLD2) activity by phosphorylation- dephosphorylation. Mol Cell Biol. 2010 Feb 22. [Epub ahead of print] IF:6.188 23. Hua Li, Feng Xu, Jin-Bao Gu and Xiao-Guang Chen*.A Severe Eosinophilic Meningoencephalitis Caused by Infection of Angiostrongylus cantonensis.Am J Trop Med HygOctober 2008 vol. 79 no. 4 568-570 24. Lei LUO, Hui-ying LIANG , Yu-shan HU, Wei-jia LIU, Yu-lin WANG, Qin-long Jing, Xue-li ZHENG*, Zhi-cong YANG* Epidemiologic,Virologic and Entomologic Characteristics of Dengue from 1978 to 2009 in Guangzhou, China. JOURNAL OF VECTOR ECOLOGY,2011,37(1) 25. Zhou Xiaohong, Wu Jinya, Huang Xiaoqin, Santhosh PK, Zhu Xingquan, Chen Xiaoguang*. Identification and charaterization of Schistosoma japonicum Sjp40, a potential antigen candidate for the early diagnosis of schistosomiasis.Diagnostic Microbiology & Infectious Diseases,2010,67,337-345. 26. Isaacs AT, Li FW, Jasinskiene N, Chen XG, Nirmala X, Marinotti O, Vinetz JM, James AA*. Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi. PLoS Pathogen, 2011,7(4)：1-13 27. Diao YP, Qi YM, Ma YJ, Xia A, Sharakhov I, Chen XG, Ling EJ, Biedler J, Tu ZJ. Next-generation Sequencing Reveals Recent Horizontal Transfer of a DNA Transposon between Divergent Mosquitoes. PLoS ONE, 2011,6(2):1-8 28. Tan F, Hu X, Luo FJ, Pan CW, Chen XG *, Induction of protective Th1 immune responses in mice by vaccination with recombinant Toxoplasma gondii nucleoside triphosphate hydrolase-II. Vaccine, 2011,29：742-2748 29. Gu JB, Liu M, Deng YH, Peng HJ and Chen XG *, Development of an Efficient Recombinant Mosquito Densovirus-Mediated RNA Interference System and Its Preliminary Application in Mosquito Control. PLoS One, 2011,6(6)：1-10 30. Wang T, Liu M, Gao XJ, Zhao Z J, Chen XG, Lun ZR*.Toxoplasma gondii: The effects of infection at different stages of pregnancy on the offspring of mice. Experiemental Parasitology, 2011,127：107-112 31. Wu JY , Lun ZR, James AA, Chen XG *. Dengue Fever in Mainland China. The American Society of Tropical Medicine and Hygiene, 2010,3(3)；664–671. 32. Gu Jinbao, Dong YQ, Peng Hongjuan, Chen XG *. A recombinant AeDNA containing the insect-specific toxin, BmK IT1, displayed an increasing pathogenicity on Aedes albopictus. The American Society of Tropical Medicine and Hygiene, 2010, 3(3)：614-623. 33. Wu JY, Zheng PM, Tu Z, Chen XG *. Identification of miRNAs in Aedes.albopictus and determination of their expression profiles during developmental stages and blood feeding using Solexa sequencing. International Journal of Infectious Diseases, 2010 , 14(S2)：14-15. 34. Zhou XH, Wu JY, Huang XQ, Kunnon SP, Zhu XQ, Chen XG *. Identification and characterization of Schistosoma japonicum Sjp40, a potential antigen candidate for the early diagnosis of schistosomiasis. Diagnostic Microbiology and Infectious disease, 2010 , 7(4):7-345. 35. Tan F, Hu X, Pan CW, Ding JQ, Chen XG *. Monoclonal antibodies against nucleoside triphosphate hydrolase-II can reduce the replication of Toxoplasma gondii. Parasitol In.,2010 , (2):41-146. 36. Hu YH , Luo L, Liu WJ. and Chen XG. Sequence analysis of the groEL gene and its potential application in identification of pathogenic bacteria. African Journal of Microbiology Research, 2010 ,4(16); 1733-1741 37. Santhosh PK, DengYH, Gu WD, Chen XG. Heterologous expression in transgenic mosquitos. Asian Pacific Journal of Tropical Medicine, 2010 , 160-166 38. Zheng XL, HuJL, SanthoshPK, Chen XG. Idengtification of necrophagous fly species from 12 different cities in China using ISSR and SCAR markers. Asian Pacific Journal of Tropical Medicine, 2010 , 510-514 39. Chen XG, Gu JB. The construction of a RNAi vector based on mosquito densovirus and its preliminary application in mosquito control. International Journal of Infectious Diseases.2010 ,14(S2):6-97 40. Wu K, Chen XG*, Li H, Yan H, Yang PL, Lun ZR, Zhu X.Q. Diagnosis of human toxoplasmosis by using the recombinant truncated SAG1 of Toxoplasma gondii. Diagn Microbiol Infect Dis.2009, 64(3);261-266. 41. Zhang H, Zhou D.H, Zhou P, Lun Z.R, Chen XG, Lin R.Q, Yuan Z.G, Zhu X.Q. Seroprevalence of Toxoplasma gondii infection in stray and household cats in Guangzhou, China. Zoonoses Public Health. 2009,56(9-10):02-505. |
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3 成果专利 1．血吸虫优势抗原的筛选和快速侦检体系的建立及其在南方战区血防工作中的应用 3．血吸虫新基因的鉴定与优势抗原的筛选分析 4．便携式血吸虫尾蚴浓集器 5．一种广州管圆线虫病的诊断抗原分子 6．一种中华按蚊转基因体系 7．一种酶标板防错加样座 8．一种基于重组抗原的弓形虫检测试剂盒 9．一种转基因番茄高效稳定表达体系 10．一种生物杀虫剂筛选模型及其制备方法和应用 11．一种便携式驱蚊帖 12．弓形虫主要表面抗原的克隆、表达及其在诊断和疫苗研制中的应用 13．一种新型的日本血吸虫优势抗原分子 |
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