学院机构

主要研究方向：

1. 人类病毒抗体筛选制备、抗病毒机制及抗体治疗研究

重要病原微生物感染引起的各种疾病严重威胁人类健康与生命，亟需深入研制特效药物、诊断试剂及疫苗。本实验室开展相关病原体的单克隆抗体筛选制备、抗病原机制研究（包含动物源性及全人源性单抗）；恢复期多抗血浆治疗人类重要病原感染的治疗效果及治疗机制研究；人类重要病原抗体诊断试剂及疫苗研究。力图制备出一系列具国际领先水平的特异性靶向人类重要病原的抗体药物，精确阐明其抗原表位及抗病毒的具体机制，为研制病原体的疫苗、治疗性药物和诊断试剂提供支持。

2. 新发传染病防治的研究

新发传染病是指近年来由新发现的新种或新型病原微生物引起的传染病，防治难度大，影响因素复杂。本实验室以SARS冠状病毒、MERS冠状病毒、新型冠状病毒、寨卡病毒、登革病毒、猴痘病毒、禽流感病毒、EV71病毒、大肠杆菌O157:H7、炭疽芽孢杆菌、副溶血性弧菌等为研究对象，开展分子溯源、致病机理、检测技术、消毒方法、疫苗构建等方面的研究。力图在感染性克隆构建技术、抗体依赖的感染增强作用（ADE）机制、新型光动力杀菌技术等研究方面形成特色，为新发传染病防治提供技术支持。

3. 实验室生物安全和转基因生物安全的研究

开展了广东省生物安全管理体系建立研究、虚拟现实生物安全实验室构建研究、正压医用安全防护头罩和便携式气密隔离保护罩的开发及应用研究、食品中转基因成分的检测技术研究。

主要课题

代表性论文

1. Li M*, Ren Z*, Wang Y*, Jiang Y*, Yang M*, Li D*, Chen J*, Liang Z, Lin Y, Zeng Z, Xu R, Wang Y, Zhu L, Xiao W, Wu Q, Zhang B, Wan C, Yang Y, Wu B, Peng J#, Zhao W#, Shen C#. Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus. Emerg Microbes Infect. 2023 Dec;12(2):2223669. doi: 10.1080/22221751.2023.2223669. PMID: 37288876; PMCID: PMC10286687.（IF，13.2，中科院一区）

2. Ouyang F, Zhang H, Jiang Y, et al. Humoral immune response characteristics of the elderly, children and pregnant women after XBB infection. J Infect. 2023;87(6):e96-e99. doi:10.1016/j.jinf.2023.10.006 (中科院小类一区，IF= 28.2).

3. Li B, Wang W, Zhao L, et al. Aggregation-Induced Emission-Based Macrophage-Like Nanoparticles for Targeted Photothermal Therapy and Virus Transmission Blockage in Monkeypox. Adv Mater. Published online November 6, 2023. doi:10.1002/adma.202305378 (中科院小类一区，IF= 29.4).

4. Wang W, Li B, Wu Y, et al. Macrophage-derived biomimetic nanoparticles for light driven theranostics towards mpox. Matter, Accepted (中科院小类一区，IF= 18.9, Cell子刊).

5. Tian Y, Li M, Yang Y, et al. An MPXV mRNA-LNP vaccine candidate elicits protective immune responses against monkeypox virus[J]. Chinese Chemical Letters, 2023: 109270 (中科院小类二区，大类一区，IF= 9.1).

6. Jiang Y, Ren Z, Yang Y, et al. Various mpox recombinant subunits induce broadly reactive antibodies against orthopoxvirus in mice. J Med Virol. 2023;95(8):e28983. doi:10.1002/jmv.28983 (中科院小类三区， IF= 12.7).

7. Zhai L, Zhang L, Jiang Y, et al. Broadly neutralizing antibodies recognizing different antigenic epitopes act synergistically against the influenza B virus. J Med Virol. 2023;95(1):e28106. doi:10.1002/jmv.28106 (中科院小类三区， IF= 12.7).

8. Liang J, Zhai L, Liang Z, et al. Rational Design and Characterization of Trispecific Antibodies Targeting the HIV-1 Receptor and Envelope Glycoprotein. Vaccines, Accepted (中科院小类三区，IF= 7.8).

9. Wen Y#, Xie Qian#, Zhang R, Zhao JB, Liu XL, Wu J, Huang YX, Yu JH, Liang RR, Qin ZR, Zeng Yan, He HT, Wang XY, Wu QH, Wan CS, Zhang B, Zhao Wei*. Pulmonary tuberculosis screening in emigrants and travellers: A retrospective analysis of Guangzhou Port in China. Travel Med Infect Dis. 2022 May 23，49: 102357.doi: 10.1016/j.tmaid.2022.102357. （IF，20.441）

10. Liu XL, Liu YF, Wu H, He ZH, Li Z, Qin ZR, Yu JH, Zhu L, Wu QH, Xiao WW, Shen CG, Wan CS, Zhang B*, Zhao W*. Murine diabetic models for dengue virus infection. J Med Virol. 2022 Dec，94(12):5943-5953. doi: 10.1002/jmv.28088. Epub 2022 Sep 12.PMID: 36000451 （IF20.693）

11. Linlin Zhai, Limin Zhang, Yushan Jiang, Baisheng Li, Minghui Yang, Khrustalev Vladislav Victorovich, Khrustaleva Tatyana Aleksandrovna, Mengjun Li, Yuelin Wang, Dong Huang, Zhujun Zeng, Zuning Ren, Hua Cao, Li Zhu, Qinghua Wu, Weiwei Xiao, Bao Zhang, Chengsong Wan, Fuxiang Wang, Ningshao Xia, Wei Zhao*, Yixin Chen*, Chenguang Shen*. Broadly neutralizing antibodies recognizing different antigenic epitopes act synergistically against the influenza B virus. J Med Virol. 2022 Aug 30. doi: 10.1002/jmv.28106. （IF20.693）

12. Xiaoxia Li#, Xuan Wan#, Jinyue Liu, Haiying Wang, Anan Li, Changwen Ke, Shixing Tang, Wei Zhao*, Shaoxi Cai, Chengsong Wan*. Luciferase Immunosorbent Assay Based on Multiple E Antigens for the Detection of Chikungunya virus-Specific IgG Antibodies Microbiology Spectrum. 2022 Apr 27，10(2):e0149621.（doi: 10.1128/spectrum.01496-21）（IF，9.043）

13. Xu Z, Li B, Jiang Y, et al. Development of a quadruple qRT-PCR assay for simultaneous identification of hypervirulent and carbapenem-resistant Klebsiella pneumoniae. Microbiol Spectr. Published online December 7, 2023. doi:10.1128/spectrum.00719-23 (中科院小类一区，IF= 3.7).

14. Liu XL#, Liang ZX#, Duan HW#, Yu JH#, Qin ZR, Li JH, Zhu L, Wu QH, Xiao WW, Shen CG, Wan CS, Wu KF, Ye H*, Zhang B*, Zhao W*. Dengue virus is involved in insulin resistance via the downregulation of IRS-1 by inducing TNF-α secretion. Biochim Biophys Acta Mol Basis Dis. 2022 Jun 22，1868(10): 166472. doi: 10.1016/j.bbadis.2022.166472. （IF6.633）

15. Yu JH#, Li XJ#, Zhou DR #, Liu XL#,He XE, Huang SH, Wu QH, Zhu L, Yu LZ, Yao JX, Zhang B*, Zhao W*. Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain Barrier. Front. Cell. Infect. Microbiol., 2022 Mar 30，12:868407. https://doi.org/10.3389/fcimb.2022.868407（IF，6.073）

16. Chen JY, Lu ZX, Yang XW, Zhou YZ, Gao J, Zhang SH, Huang S, Cai JT, Yu JH, Zhao W*, Zhang B*. Severe Acute Respiratory Syndrome Coronavirus 2 ORF8 Protein Inhibits Type I Interferon Production by Targeting HSP90B1 Signaling. Front. Cell. Infect. Microbiol., 23 May 2022. https://doi.org/10.3389/fcimb.2022.899546（IF，6.073）

17. Yu JH#, Qin ZR#, Liu XL#, He XE#, Yao JX, Zhou X, Wen K, Yu N, Wu QH, Xiao WW, Zhu L, Wan CS, Zhang B*, Zhao W*. High-specificity targets in SARS-CoV-2 N protein for serological detection and distinction from SARS-CoV, Computers in Biology and Medicine, 2022, 143,105272.(IF: 6.698)

18. Qin ZR#, Chen YY#, Yu JH#, He XE#, Liu XL, Zhu L, Wu QH, Wan CS, Zhang B *and Zhao W*. Rapid Construction of an Infectious Clone of the Zika Virus, Strain ZKC2. Front. Microbiol. 2021, 12: 752578（IF 5.640）

19. Liang ZX, Liu XL, Qin ZR, Li JS, Yu JH, He XE, Zhu L, Fan HY, Hu YS, Yao JX, Shen CG, Wan CS, Zhang B*, Zhao W*. Photodynamic inactivation of Shigella flexneri by curcumin. LWT，2022，153: 112491(IF 6.056)

20. Lin Y, Sun Q, Zhang B, Zhao W, Shen C. The regulation of lncRNAs and miRNAs in SARS-CoV-2 infection. Front Cell Dev Biol. 2023;11:1229393. Published 2023 Jul 27. doi:10.3389/fcell.2023.1229393 (中科院小类二区， IF= 5.5).

21. Ren Z, Shen C, Peng J. Status and Developing Strategies for Neutralizing Monoclonal Antibody Therapy in the Omicron Era of COVID-19. Viruses. 2023;15(6):1297. Published 2023 May 31. doi:10.3390/v15061297 (中科院小类二区， IF= 4.7).

22. Chen TT, He XE, Zhang PR, Yuan YW, Lang XY, Yu JH, Qin ZR, Li XJ, Zhang QW, Zhu L, Zhang B, Wu QH*, Zhao W*. Research advancements in the neurological presentation of flaviviruses. Rev Med Virol, 2018，e2021. (IF 5.034)

23. Wu QH, Cao ZX, Xiao WW, Zhu L, Xie Q, Li L, Zhang B*, Zhao W*. Study on Therapeutic Action and Mechanism of TMZ Combined with RITA Against Glioblastoma. Cell Physiol Biochem 2018, 51(6) : 2536-2546. (IF 5.500)

24. Xie Q#, He XY#, Yang FJ, Liu XL, Li Y, Liu YJ, Yang ZM, Yu JH, Zhang B*, Zhao W*. Analysis of the Genome Sequence and Prediction of B-Cell Epitopes of the Envelope Protein of Middle East Respiratory Syndrome-Coronavirus. IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2018, 15(4):1344-1350. （IF, 2.428)

25. Zhang Z, Sun M, Deng J, Yu J, Yang X, Zhao W#, Chen G# and Wang P#(2019) Zika Virus Induced More Severe Inflammatory Response Than Dengue Virus in Chicken Embryonic Livers. Front. Microbiol. 10:1127. doi: 10.3389/fmicb.2019.01127 (IF, 4.259)

26. Yang YC#, Lyu TB#, Zhou RN#, He XE#, Ye KY, Xie Q, Zhu L, Chen TT, Shen C, Wu QH, Zhang B*, Zhao W*.The Antiviral and Antitumor Effects of Defective Interfering Particles/Genomes and Their Mechanisms. Front. Microbiol., 2019， 10: 1852. 09 August 2019 https://doi.org/10.3389/fmicb.2019.01852 (IF, 4.259)

27. Luo SX#, Zhao W#, Ma XR, Zhang PL, Liu BC, Zhang L, Wang WJ, Wang YZ, Fu YS, Allain JP, Li TT*, Li CY*. A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. PLoS Negl Trop Dis 2020, 14(2): e0008027. (IF, 4.411)

28. Lu WZ#, Shi LJ#, Gao J, Zhu HM, Hua Y, Cai JT, Wu XB, Wan CS, Zhao W*, Zhang B*. Piperlongumine Inhibits Zika Virus Replication In vitro and Promotes Up-Regulation of HO-1 Expression, Suggesting An Implication of Oxidative Stress. Virol Sin. 2021,36(3):510-520. doi: 10.1007/s12250-020-00310-6. Epub 2020 Nov 13. (IF, 4.3268)

29. Zhu Q, Lin Q, Jiang Y, et al. Construction and application of the conditionally essential gene knockdown library in Klebsiella pneumoniae to screen potential antimicrobial targets and virulence genes via Mobile-CRISPRi-seq. Appl Environ Microbiol. 2023;89(10):e0095623. doi:10.1128/aem.00956-23 (中科院小类二区， IF= 4.4).

30. Yang Y, Zhang J, Zhang S, et al. A novel nanobody broadly neutralizes SARS‐CoV‐2 via induction of spike trimer dimers conformation[C]//Exploration. 2023: 20230086. (新杂志，2024年出首个影响因子).

31. J. Ou, W. Lan, X. Wu, T. Zhao, B. Duan, P. Yang, Y. Ren, L. Quan, W. Zhao, D. Seto, J. Chodosh, Z. Luo, J. Wu, and Q. Zhang, Tracking SARS-CoV-2 Omicron diverse spike gene mutations identifies multiple inter-variant recombination events. Signal Transduction and Targeted Therapy. 2022/04/26; 7(1):138 doi: 10.1038/s41392-022-00992-2. IF：38.104 一区

32. Jiang Y, Zhang H, Yu J, Huang D, Zhai L, Li M, Wang Y, Ren Z, Zou L, Zheng Z, Hu H, Zhang J, Zhang B, Zhao W, Yang X, Li B, Shen C. Humoral immune response to authentic circulating severe acute respiratory syndrome coronavirus 2 variants elicited by booster vaccination with distinct receptor-binding domain subunits in mice. J Med Virol. 2022 Sep;94(9):4533-4538. doi: 10.1002/jmv.27882. （IF 20.693，中科院小类三区）。

33. Zou L, Zhang H, Zheng Z, Jiang Y, Huang Y, Lin S, Yu J, Deng X, He J, Shen C, Li B. Serosurvey in SARS-CoV-2 inactivated vaccine-elicited neutralizing antibodies against authentic SARS-CoV-2 and its viral variants. J Med Virol. 2022 Dec;94(12):6065-6072. doi: 10.1002/jmv.28049. （IF 20.693，中科院小类三区）。

34. Li M, Wang Y, Li C, Xu R, Chen J, Zhang J, Yang M, Jiang Y, Li Y, Zeng Z, Wu Z, Zhao W, Wu B, Shen C. Development of monoclonal antibody-based antigens detection assays for orthopoxvirus and monkeypox virus. J Infect. 2022 Dec;85(6):702-769. doi: 10.1016/j.jinf.2022.10.036. （IF 38.637，中科院小类一区TOP）。

奖励：

1. 赵卫、江丽芳、龙北国、晏辉钧、张文炳、方丹云、胡族琼。《我国广东地区SARS冠状病毒分离株病原学的基础研究》，中国人民解放军总后勤部，军队科技进步三等奖。

2. 46人并列排名，《广东省传染性非典型肺炎（SARS）防治研究》，广东省人民政府，广东省科技进步特等奖。

3. 万成松，王虹，曾位森，王省良，龙北国，华旭初，曹 虹，张文炳，陈金华，刘丹。PCR微板核酸杂交技术在病原微生物核酸诊断、基因分型和基因突变中的应用。广东省科技进步二等奖。